Biomedical Mass Spectrometry Core

TJG instrumentPart of the Southeast Center for Integrated Metabolomics, this core facility specializes in the development of assays for the quantification of small molecules in complex media (plasma, serum, urine, tissues, cell cultures, etc) using mass spectrometry for both human and animal studies. The staff of the core consists of 3 Ph.D. scientists with backgrounds in instrumentation for mass spectrometry, physical chemistry, biochemistry, and nutrition.

We can develop a new assay for you or apply one of our previously developed assays. Examples of quantitative assays developed for research purposes are uric acid metabolites, tryptophan metabolites, one-carbon related metabolites (14 total compounds), DNA/RNA oxidation markers, DNA methylation, Coenzyme Q9 andQ10, Cholesterol, and rapamycin. In addition, we have developed assays for the semi-quantitative analysis of many lipid classes such as phosphatidylcholines and sphingomyelins, phosphatidylethanolamines, phosphatidyleserines, cerebrosides, and ceramides.

If you are writing a grant, we are happy to meet with you to discuss the options available for analysis as well as provide perspective on new analytical tools that could be used for your research study, such as imaging mass spectrometry, where we map specific chemical signatures within an excised tissue sectioned and mounted for MALDI-MS (in collaboration with the Department of Chemistry).

Finally, we can also train a member of your laboratory in the operation of our instruments and provide access to the instruments and assistance in method development once they are trained.

Instruments in the core are: Thermo TSQ Quantum Ultra with Accela UPLC and autosampler, Thermo TSQ Quantum access with UPLC and autosampler, Thermo LTQ Velos with UPLC and autosampler, Thermo TSQ 7000 with Agilent 1100 HPLC and autosampler, Thermo LCQ Deca with Agilent 1100 HPLC and autosampler, Thermo DSQ GC/MS with autosampler, and an Agilent 5973 GC/MS with autosampler.

Please contact Tim Garrett, Ph.D., or 273-5050