Genotyping Core

At the CTSI Genotyping Core, we provide consultation and guidance to the researchers who need assistance in designing and performing genetics and pharmacogenetics studies, and provide genotype data for their research projects. Services are offered as fee for service (and in some circumstances, through collaboration) to many researchers working on human, plant, small and large animal genetics.

Two units comprise the CTSI Genotyping Core, the Center for Pharmacogenomics Genotyping Laboratory, and the Molecular Services Core of the ICBR. The Center for Pharmacogenomics Genotyping Laboratory provides sample handling and processing services, along with individualized single-plex through 384-plex genotyping assays. The ICBR provides sequencing and large-scale (e.g. genome-wide) genotyping services.

Services include; receiving and processing of different types of samples used for DNA and RNA isolation (eg. Blood, different human, animal, and plant tissues, mouthwash, saliva), Whole Genome Amplification of DNA, DNA quantitation by spectrophotometer; DNA quantitation by PicoGreen, PCR and RT-PCR reactions, measuring telomere length and telomerase activity, mutation discovery, genotyping by conventional RFLP method and low to high-throughput genotyping techniques using the platforms that are available at the UF Center for Pharmacogenomics such as; Pyrosequencing, TaqMan, and Illumina Veracode BeadXpress.

Next Generation Sequencing and genotyping groups at ICBR offers cutting-edge DNA sequencing services on the Roche 454 Genome Sequencer (GS) FLXTM Standard, FLX TitaniumTM and Applied Biosystems (ABI) SOLiDTM instruments and genotyping services such as, Whole genome SNP genotyping , Copy number/Loss of heterozygosity genotyping, standard panels (cancer SNP, non-synonymous human, MHC), Human and mouse linkage analysis panels, GoldenGate custom panels, iSelect custom panels on Illumina BeadStation 500G Genotyping System.

Detailed information on services can be obtained at, http://www.cop.ufl.edu/research/research-centers/center-for-pharmacogenomics/ and http://www.biotech.ufl.edu/services.html

Contact

For more information, contact Taimour Langaee, Ph.D., langaee@cop.ufl.edu or (352)273-6357 (Center for Pharmacogenomics) or William Farmerie, Ph.D., wgf@biotech.ufl.edu or (352)-273-8049 (ICBR Molecular Services Core)