2020 – 2022 TDDC Publications

See below for a list of publications that highlight research that was conducted with the support of the CTSI’s Translational Drug Development Core.


Berthold EC, Kamble SH, Raju KS, Kuntz MA, Senetra AS, Mottinelli M, León F, Restrepo LF, Patel A, Ho NP, Hiranita T, Sharma A, McMahon LR, McCurdy CR. The Lack of Contribution of 7-Hydroxymitragynine to the Antinociceptive Effects of Mitragynine in Mice: A Pharmacokinetic and Pharmacodynamic Study. Drug Metab Dispos. 2022 Feb;50(2):158-167. doi: 10.1124/dmd.121.000640. Epub 2021 Nov 10. PMID: 34759012; PMCID: PMC8969138.

Hiranita T, Obeng S, Sharma A, Wilkerson JL, McCurdy CR, McMahon LR. In vitro and in vivo pharmacology of kratom. Adv Pharmacol. 2022;93:35-76. doi: 10.1016/bs.apha.2021.10.001. Epub 2021 Dec 13. PMID: 35341571.

Kamble SH, Berthold EC, Kanumuri SRR, King TI, Kuntz MA, León F, Mottinelli M, McMahon LR, McCurdy CR, Sharma A. Metabolism of Speciociliatine, an Overlooked Kratom Alkaloid for its Potential Pharmacological Effects. AAPS J. 2022 Jul 19;24(5):86. doi: 10.1208/s12248-022-00736-8. PMID: 35854066.

Leksungnoen N, Andriyas T, Ngernsaengsaruay C, Uthairatsamee S, Racharak P, Sonjaroon W, Kjelgren R, Pearson BJ, McCurdy CR, Sharma A. Variations in mitragynine content in the naturally growing Kratom (Mitragyna speciosa) population of Thailand. Front Plant Sci. 2022 Oct 27;13:1028547. doi: 10.3389/fpls.2022.1028547. PMID: 36388525; PMCID: PMC9648690.

LeSaint KT, Yin S, Sharma A, Avery BA, McCurdy CR, Waksman JC. Acute Renal Insufficiency Associated With Consumption of Hydrocodone- and Morphine-Adulterated Kratom (Mitragyna Speciosa). J Emerg Med. 2022 Jul;63(1):e28-e30. doi: 10.1016/j.jemermed.2022.02.004. Epub 2022 Aug 6. PMID: 35940982.

Mamallapalli J, Kanumuri SRR, Corral P, Johnston E, Zhuang C, McCurdy CR, Mathews CA, Sharma A, Xing C. Characterization of Different Forms of Kava (Piper methysticum) Products by UPLC-MS/MS. Planta Med. 2022 Nov;88(14):1348-1359. doi: 10.1055/a-1708-1994. Epub 2021 Nov 28. PMID: 34839465.

Popa R, Kamble SH, Kanumuri RS, King TI, Berthold EC, Intagliata S, Sharma A, McCurdy CR. UPLC-MS/MS method for the quantification of MCI-77, a novel sigma-1 receptor ligand, and its application to pharmacokinetic studies. J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Apr 30;1196:123187. doi: 10.1016/j.jchromb.2022.123187. Epub 2022 Mar 5. PMID: 35278810.

Zhang M, Sharma A, León F, Avery B, Kjelgren R, McCurdy CR, Pearson BJ. Plant growth and phytoactive alkaloid synthesis in kratom [Mitragyna speciosa (Korth.)] in response to varying radiance. PLoS One. 2022 Apr 26;17(4):e0259326. doi: 10.1371/journal.pone.0259326. PMID: 35472200; PMCID: PMC9041851.


Chear NJ, León F, Sharma A, Kanumuri SRR, Zwolinski G, Abboud KA, Singh D, Restrepo LF, Patel A, Hiranita T, Ramanathan S, Hampson AJ, McMahon LR, McCurdy CR. Exploring the Chemistry of Alkaloids from Malaysian Mitragyna speciosa (Kratom) and the Role of Oxindoles on Human Opioid Receptors. J Nat Prod. 2021 Apr 23;84(4):1034-1043. doi: 10.1021/acs.jnatprod.0c01055. Epub 2021 Feb 26. PMID: 33635670; PMCID: PMC8693998.

Kamble SH, Berthold EC, King TI, Raju Kanumuri SR, Popa R, Herting JR, León F, Sharma A, McMahon LR, Avery BA, McCurdy CR. Pharmacokinetics of Eleven Kratom Alkaloids Following an Oral Dose of Either Traditional or Commercial Kratom Products in Rats. J Nat Prod. 2021 Apr 23;84(4):1104-1112. doi: 10.1021/acs.jnatprod.0c01163. Epub 2021 Feb 23. PMID: 33620222; PMCID: PMC8694001.

León F, Obeng S, Mottinelli M, Chen Y, King TI, Berthold EC, Kamble SH, Restrepo LF, Patel A, Gamez-Jimenez LR, Lopera-Londoño C, Hiranita T, Sharma A, Hampson AJ, Canal CE, McMahon LR, McCurdy CR. Activity of Mitragyna speciosa (“Kratom”) Alkaloids at Serotonin Receptors. J Med Chem. 2021 Sep 23;64(18):13510-13523. doi: 10.1021/acs.jmedchem.1c00726. Epub 2021 Sep 1. PMID: 34467758; PMCID: PMC9235362.

Maxwell EA, King TI, Kamble SH, Raju KSR, Berthold EC, León F, Hampson A, McMahon LR, McCurdy CR, Sharma A. Oral Pharmacokinetics in Beagle Dogs of the Mitragynine Metabolite, 7-Hydroxymitragynine. Eur J Drug Metab Pharmacokinet. 2021 May;46(3):459-463. doi: 10.1007/s13318-021-00684-2. Epub 2021 Apr 13. PMID: 33847897; PMCID: PMC8179724.

Ostrov DA, Bluhm AP, Li D, Khan JQ, Rohamare M, Rajamanickam K, K Bhanumathy K, Lew J, Falzarano D, Vizeacoumar FJ, Wilson JA, Mottinelli M, Kanumuri SRR, Sharma A, McCurdy CR, Norris MH. Highly Specific Sigma Receptor Ligands Exhibit Anti-Viral Properties in SARS-CoV-2 Infected Cells. Pathogens. 2021 Nov 20;10(11):1514. doi: 10.3390/pathogens10111514. PMID: 34832669; PMCID: PMC8620039.

Sharma A, McCurdy CR. Assessing the therapeutic potential and toxicity of Mitragyna speciosa in opioid use disorder. Expert Opin Drug Metab Toxicol. 2021 Mar;17(3):255-257. doi: 10.1080/17425255.2021.1853706. Epub 2020 Dec 11. PMID: 33213215.

Tadjalli A, Seven YB, Sharma A, McCurdy CR, Bolser DC, Levitt ES, Mitchell GS. Acute morphine blocks spinal respiratory motor plasticity via long-latency mechanisms that require toll-like receptor 4 signalling. J Physiol. 2021 Aug;599(15):3771-3797. doi: 10.1113/JP281362. Epub 2021 Jul 6. PMID: 34142718; PMCID: PMC8437142.


Behnood-Rod A, Chellian R, Wilson R, Hiranita T, Sharma A, Leon F, McCurdy CR, McMahon LR, Bruijnzeel AW. Evaluation of the rewarding effects of mitragynine and 7-hydroxymitragynine in an intracranial self-stimulation procedure in male and female rats. Drug Alcohol Depend. 2020 Oct 1;215:108235. doi: 10.1016/j.drugalcdep.2020.108235. Epub 2020 Aug 18. PMID: 32889450; PMCID: PMC7542979.

Berthold EC, Kamble SH, Raju KS, King TI, Popa R, Sharma A, León F, Avery BA, McMahon LR, McCurdy CR. Preclinical pharmacokinetic study of speciociliatine, a kratom alkaloid, in rats using an UPLC-MS/MS method. J Pharm Biomed Anal. 2021 Feb 5;194:113778. doi: 10.1016/j.jpba.2020.113778. Epub 2020 Nov 21. PMID: 33277117.

Berthold EC, Yang R, Sharma A, Kamble SH, Kanumuri SR, King TI, Popa R, Freeman JH, Brym ZT, Avery BA, McCurdy CR. Regulatory sampling of industrial hemp plant samples (Cannabis sativa L.) using UPLC-MS/MS method for detection and quantification of twelve cannabinoids. J Cannabis Res. 2020 Dec 10;2(1):42. doi: 10.1186/s42238-020-00050-0. PMID: 33526142; PMCID: PMC7819288.

Kamble SH, Sharma A, King TI, Berthold EC, León F, Meyer PKL, Kanumuri SRR, McMahon LR, McCurdy CR, Avery BA. Exploration of cytochrome P450 inhibition mediated drug-drug interaction potential of kratom alkaloids. Toxicol Lett. 2020 Feb 1;319:148-154. doi: 10.1016/j.toxlet.2019.11.005. Epub 2019 Nov 7. PMID: 31707106; PMCID: PMC7902086.

Kamble SH, León F, King TI, Berthold EC, Lopera-Londoño C, Siva Rama Raju K, Hampson AJ, Sharma A, Avery BA, McMahon LR, McCurdy CR. Metabolism of a Kratom Alkaloid Metabolite in Human Plasma Increases Its Opioid Potency and Efficacy. ACS Pharmacol Transl Sci. 2020 Jul 31;3(6):1063-1068. doi: 10.1021/acsptsci.0c00075. PMID: 33344889; PMCID: PMC7737207.

King TI, Sharma A, Kamble SH, León F, Berthold EC, Popa R, Cerlati O, Prentice BM, McMahon LR, McCurdy CR, Avery BA. Bioanalytical method development and validation of corynantheidine, a kratom alkaloid, using UPLC-MS/MS, and its application to preclinical pharmacokinetic studies. J Pharm Biomed Anal. 2020 Feb 20;180:113019. doi: 10.1016/j.jpba.2019.113019. Epub 2019 Dec 5. PMID: 31838282; PMCID: PMC7350276.

Maxwell EA, King TI, Kamble SH, Raju KSR, Berthold EC, León F, Avery BA, McMahon LR, McCurdy CR, Sharma A. Pharmacokinetics and Safety of Mitragynine in Beagle Dogs. Planta Med. 2020 Nov;86(17):1278-1285. doi: 10.1055/a-1212-5475. Epub 2020 Jul 21. PMID: 32693425; PMCID: PMC7907416.

Obeng S, Wilkerson JL, León F, Reeves ME, Restrepo LF, Gamez-Jimenez LR, Patel A, Pennington AE, Taylor VA, Ho NP, Braun T, Fortner JD, Crowley ML, Williamson MR, Pallares VLC, Mottinelli M, Lopera-Londoño C, McCurdy CR, McMahon LR, Hiranita T. Pharmacological Comparison of Mitragynine and 7-Hydroxymitragynine: In Vitro Affinity and Efficacy for μ-Opioid Receptor and Opioid-Like Behavioral Effects in Rats. J Pharmacol Exp Ther. 2021 Mar;376(3):410-427. doi: 10.1124/jpet.120.000189. Epub 2020 Dec 31. PMID: 33384303; PMCID: PMC7923387.

Pearson BJ, Campbell S, Avery B, Mccurdy C, Francisco J, Sharma A, Kjelgren R. (2020). Preliminary examination of mitragynine and 7-hydroxymitragynine synthesis in response to production environment and postharvest techniques of Mitragyna speciosa. Acta Horticulturae. 89-96. 10.17660/ActaHortic.2020.1274.10.

Popa R, Kamble SH, Kanumuri RS, King TI, Berthold EC, Intagliata S, Sharma A, McCurdy CR. Bioanalytical method development and pharmacokinetics of MCI-92, a sigma-1 receptor ligand. J Pharm Biomed Anal. 2020 Nov 30;191:113610. doi: 10.1016/j.jpba.2020.113610. Epub 2020 Sep 13. PMID: 32971495.

Zhang M, Sharma A, León F, Avery B, Kjelgren R, McCurdy CR, Pearson BJ. Effects of Nutrient Fertility on Growth and Alkaloidal Content in Mitragyna speciosa (Kratom). Front Plant Sci. 2020 Dec 21;11:597696. doi: 10.3389/fpls.2020.597696. PMID: 33408731; PMCID: PMC7779599.